What is high-throughput microscopy?

What is high-throughput microscopy?

High throughput imaging is a term used to describe automated light microscopy and image analysis of large numbers of samples. It is a useful technique for phenotypic screening assays, evaluating the response of cells, cell-based models, or organisms to various types of perturbagens.

What is considered high throughput screening?

High-throughput screening (HTS) is a drug discovery process that allows automated testing of large numbers of chemical and/or biological compounds for a specific biological target, for example through binding assays.

What is high-throughput analysis?

High-throughput (HT) analysis is becoming more and more important. It means analysis of dozens, hundreds, or even thousands of samples per day in a given laboratory or on a particular instrument.

Why is high throughput screening necessary?

The main goal of the HTS technique is to accelerate drug discovery by screening large compound libraries at a rate that may exceed a few thousand compounds per day or per week. It is of vital importance, because parallel and combinatorial chemical synthesis generates a vast number of novel compounds.

What is high throughput imaging?

High-throughput imaging (HTI, see Glossary) refers to the use of automated microscopy and image analysis to visualize and quantitatively capture cellular features at a large scale.

What are high-throughput techniques?

High-throughput sequencing, or massively parallel sequencing, is a collection of methods and technologies that can sequence DNA thousands/millions of fragments at a time. This is in contrast to older technologies that can produce a limited number of fragments at a time.

What are high throughput technologies?

While sequencing information has traditionally been elucidated using a low throughput technique called Sanger sequencing, high throughput sequencing (HTS) technologies are capable of sequencing multiple DNA molecules in parallel, enabling hundreds of millions of DNA molecules to be sequenced at a time.

What are high-throughput technologies?

What are high throughput techniques?

What is high-throughput imaging?

What is an example of high-throughput technology?

Other technologies that enable high-throughput include automation, robotics, and liquid-handling robotics.

What are high throughput applications?

Introduction. High throughput screening (HTS), or the process by which libraries of small molecule compounds are individually assessed for binding, activating or inactivating biological activity in drug target molecules, has been part of the drug discovery process for more than two decades.

Why do we need high throughput?

The main contribution that high-throughput screening brought is that it allows to screen numerous samples at the same time, as previously stated. Consequently, the handling time is also reduced.

What is high throughput screening?

Definition High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level.

What is high-throughput high-content microscopy?

High-throughput high-content microscopy: High-quality images and high-content image analysis software are combined with high-throughput automation technologies for analyzing the results of high-throughput screening assays.

What is the Columbia Genome Center high-throughput screening?

The Columbia Genome Center’s High-Throughput Screening facility receives funding from NYSTEM, the New York State foundation to support stem cell research. We are the official core facility for screening for NYSTEM investigators throughout the state.

What is the imaging capacity of the in Cell 2000?

The instrument is equipped with a large CCD camera (resolution 2048 x 2048 pixels) that is capable of whole-well imaging in 96- or 384-well microplates. The IN Cell 2000 has several imaging modes, including image restoration and deconvolution.