What is PAS positive diastase resistant?
What is PAS positive diastase resistant?
Morphologically, the finding of PAS-positive, diastase-resistant globules in periportal hepatocytes, is the hallmark of Z-type α1-AT. (Figs 4.20, 4.21) They represent the retention of the abnormal enzyme within the rough endoplasmic reticulum or its transition zone as demonstrated by immunohistochemical methods (Fig.
What is the purpose of PAS diastase?
The primary purpose of using the PAS with Diastase staining procedure is to differentiate glycogen from other PAS positive elements such as mucin that may be present in the tissue sample.
What is the meaning of PAS positive?
It can be used to diagnose α1-antitrypsin deficiency if periportal liver hepatocytes stain positive. Aggregates of PAS-positive lymphocytes are present in epidermis in Mycosis fungoides and Sézary syndrome, called Pautrier microabscesses. Ewing sarcoma. Erythroleukemia, a leukemia of immature red blood cells.
What is DPAS stain used for?
Aims—For many years the presence of strong intracytoplasmic periodic acid Schiff (PAS) positive, diastase resistant (DPAS) staining within atypical cells has been used in this laboratory as a marker for carcinoma in fine needle aspirates from breast lesions.
What bacteria are PAS positive?
The following bacterial species were found to be consistently periodic acid-Schiff positive after diastase digestion: B cereus, Corynebacterium diphtheriae, Propionibacterium acnes, Klebsiella pneumoniae, and Micrococcus luteus.
What PAS stain kidneys?
The PAS stain is used to demonstrate polysaccharides such as glycogen, and mucosubstances such as glycoproteins, glycolipids and mucins in tissues. It is used as a replacement for the H&E in kidney pathology.
What Colour does PAS stain?
magenta
It is the basis of the PAS stain. PAS stains carbohydrates and carbohydrate rich macromolecules a deep red colour (magenta). Mucus in cells and tissues, Basement membranes, and Brush borders of kidney tubules and small and large intestines Reticular fibres (i.e. collagen) in connective tissue and Cartilage.
What color is PAS stain?
Magenta
General Principles of the Stain
| Histologic feature | Colour with PAS Stain |
|---|---|
| Glycogen, basement membranes, mucus substances, fungal organisms | Magenta |
| Nuclei | Blue (with haematoxylin counter stain) |
| Other tissue elements | Green (with light green counter stain) |
Which leukemia is PAS positive?
The PAS positivity, defined by the presence of blocks or coarse granules in 5% or more of the blasts, was found in 15 of 29 lymphoblastic leukemias and in four of the myeloblastic leukemias.
Is PAS a special stain?
From a diagnostic point of view, PAS is one of the most useful and used special stains in the pathology laboratory. It can help on the one hand to the differential diagnosis of tumors and on the other hand to detect fungal diseases.
What does PAS highlight in a renal biopsy?
Renal Pathology. This normal glomerulus is stained with PAS to highlight basement membranes of glomerular capillary loops and tubular epithelium. The capillary loops of this normal glomerulus are well-defined and thin. The endothelial cells are seen in capillary loops.