Is Coomassie stain hazardous?
Is Coomassie stain hazardous?
Material may be irritating to the mucous membranes and upper respiratory tract. May be harmful by inhalation, ingestion, or skin absorption. May cause eye, skin, or respiratory irritation.
Is Coomassie Blue used in SDS-PAGE?
Coomassie blue dyes are a family of dyes commonly used to stain proteins in SDS-PAGE gels. The gels are soaked in dye, and excess stain is then eluted with a solvent (“destaining”). This treatment allows the visualization of proteins as blue bands on a clear background.
What is the difference between Coomassie R250 and G250?
G-250: Which One Should You Use? Basically, both R-250 (R signifies the slightly reddish tint in the blue color of the dye) and G-250 (G signifies the greenish tint in the dye) have relatively high sensitivity and allow for easy detection (they develop intensely colored complexes upon binding with protein molecules).
Is Coomassie Blue stain toxic?
Coomassie Briliant Blue R-250 Dye (6104-59-2) Potential Adverse human health effects and symptoms : Non-toxic if swallowed (LD50 oral, rat > 5000 mg/kg). Not irritant to skin. Not irritant to eyes.
Can Coomassie stain go down the drain?
Environmentally friendly Coomassie Blue No fume hood is required and these can be easily disposed of down the sink. Of course you should continue to wear gloves to prevent protein gel contamination. Eco-friendly commercial stains are also mass spec compatible.
How do you dispose of Coomassie blue?
The blue-colored Kimwipes did not release the stain even when squeezed dry after incubation in various salts, water, or acid solutions for five weeks. The CBB removed thus can be simply disposed as solid waste and will not leach out from solid landfills.
What is another name for Coomassie Blue?
Coomassie brilliant blue
|Solid Coomassie brilliant blue G Brilliant blue G in isopropanol solution|
|Other names C.I. 42655, C.I. Acid Blue 90 Brilliant indocyanine G, Brillantindocyanin G Xylene Brilliant Cyanine G, Serva Blue G|
How do you stain SDS-PAGE with Coomassie Blue?
Stain: Dissolve 0.4g of Coomassie blue R350 in 200 mL of 40% (v/v) HPLC grade methanol in water with stirring as needed. Filter the solution to remove any insoluble Page 2 material. Add 200mL of 20% (v/v) acetic acid in water.
What is CBB R250?
Coomassie R-250 and G-250 dyes are two chemical forms of a disulfonated triphenylmethane compound that is commonly used as the basis of stains for detection of proteins in gel electrophoresis and Bradford-type assay reagents for protein quantitation.
How do you dispose of Coomassie Blue stain?
Can Coomassie blue go down the sink?
COOMASSIE BLUE destain may contain alcohol* and cannot be disposed of in the sink! Hazardous Materials are NEVER to be disposed of in the sink!
Can Coomassie blue go down the drain?
How do you make Coomassie Blue?
Dissolve 0.25 g of Coomassie Brilliant Blue R-250 in 90 ml of methanol:H2O (1:1, v/v) and 10 ml of glacial acetic acid. Filter the solution through a Whatman No. 1 filter to remove any particulate matter.
How do you make Coomassie Blue stain?
To prepare 1 l of a staining solution, dissolve 1 g of Coomassie R250 to 300 ml of methanol. Then add 650 ml of MQ water and 50 ml of acetic acid. Stir the solution on a magnetic stirrer for 2 h. The solution can be filtered through a Whatman No.
How long do I stain with Coomassie Blue?
Discard each rinse. Stain the mini-gel with enough Invitrogen™ SimplyBlue™ SafeStain (20-100 ml) to cover the gel. Stain for 1 hour at room temperature with gentle shaking. Bands will begin to develop within minutes.
How do you make a CBB stain?
Can Koh be poured down the drain?
Following compounds may be disposed by flushing them down the drain if there are only small quantities: Salts of light metals like calcium chloride or sodium sulfate. Mineral acids (Hydrochloric acid, sulfuric acid, phosphoric acid) Sodium hydroxide, potassium hydroxide.
How do you dispose of KOH solutions?
For Potassium Hydroxide in solution absorb liquids in dry sand, earth, or a similar material and place into sealed containers for disposal. DO NOT wash into sewer. For water spills, neutralize with dilute acid (such as Acetic Acid). Potassium Hydroxide is harmful to aquatic life in very low concentrations.
How do I prepare a solution of Coomassie R-250 dye?
How do I prepare a solution of Coomassie R-250 dye? Add 100 mL of glacial acetic acid to 450 mL distilled water. Dissolve 3 g of Coomassie R-250 dye in 450 mL methanol. Mix the acetic acid and methanol solutions.
How much protein should I load for Coomassie?
For a mini gel being stained with Coomassie brilliant blue R-250, load a maximum of 50 g for a complex sample. For a sample with only one or a few bands, load about 1 g per band. If your protein of interest is in low abundance, you may load up to 100 g of protein, but the resolution of the gel may be poor.
How do you make Coomassie Brilliant Blue solution?
Dissolve 100 mg Coomassie Brilliant Blue G-250 in 50 ml of 95% ethanol and add 100 ml of 85% phosphoric acid while stirring continuously. When the dye has dissolved dilute to 1 l in water.
Can you leave Coomassie stain overnight?
Stain cannot be re-used. Note: Gel can be stained for up to 3 hours, but after 3 hours, sensitivity will decrease. If you need to leave the gel overnight in the stain, add 2 ml of 20% NaCl (w/v) in water for every 20 ml of stain. This procedure will not affect sensitivity.
Is KOH flammable?
Potassium Hydroxide is CORROSIVE in MOIST AIR to METALS (such as ALUMINUM, ZINC, TIN and LEAD) and forms flammable and explosive Hydrogen gas.
Can I pour Coomassie down the drain?
COOMASSIE BLUE destain may contain alcohol* and cannot be disposed of in the sink!
What do I need for Coomassie blue staining?
You may use any Coomassie™ staining protocol of choice. You will need following items for Coomassie Blue Staining. 0.1% Coomassie® R-250 in 40% ethanol and 10% acetic acid (if using Invitrogen™ Coomassie™ R-250 Staining) Destaining Solution consisting of 10% ethanol and 7.5% acetic acid (if using Invitrogen™ Coomassie™ R-250 Staining)
What is QC colloidal Coomassie stain?
QC Colloidal Coomassie (161-0803) — the newest in the family of Bio-Rad Coomassie stains, QC Colloidal Coomassie G-250 allows for flexible staining and destaining times and does not require use of methanol for fixing. Low background, high sensitivity, superior reproducibility
What is the manufacturer’s code for the Coomassie G-250?
MATERIAL SAFETY DATA SHEETCOOMASSIE BLUE G-250 PRODUCT CODE NUMBER(S): 3380-5 PREVENTIVE MEASURES Engineering Controls:Local exhaust recommended. Respiratory Protection:Dust/mist mask.
How do you use simplyblue simplyblue stain?
Discard each rinse. Stain the mini-gel with enough Invitrogen™ SimplyBlue™ SafeStain (20-100 ml) to cover the gel. Stain for 1 hour at room temperature with gentle shaking. Bands will begin to develop within minutes. After incubation, discard the stain. Stain cannot be re-used.