What is antisense gene technology?
What is antisense gene technology?
Antisense DNA technology is a method to inhibit or downregulate the production of a target protein by using antisense DNA or RNA molecules. An antisense sequence is a DNA or RNA that is perfectly complementary to the target nucleotide sequence present in the cell.
Which is an example of antisense technology?
For example, an antisense oligonucleotide inhibitor of an apo-B protein is used to treat Familial Homozygous Hypercholesterolaemia. Apo-B is an essential structural component of lipoproteins. By halting the translation of apo-B, their plasma concentrations decrease, leading to a reduced risk of coronary heart disease.
What is meant by gene silencing?
Gene silencing is generally defined as an epigenetic modification of gene expression leading to inactivation of previously active individual genes or larger chromosome regions.
What is the difference between antisense and RNAi?
Definition. Antisense therapy means the selective, sequence-specific inhibition of gene expression by single-stranded DNA oligonucleotides. In contrast, RNA interference (RNAi) is triggered by double-stranded RNA (dsRNA) and causes sequence-specific mRNA degradation of single-stranded target RNAs in response to dsRNA.
How antisense technology is applicable for genetic disorders treatment?
Antisense technology might be a gateway to the treatment of diseases by targeting the expression of genes rather than permanently altering them. Antisense oligonucleotides can alter target gene expression by binding to RNA. Once bound, the ASO either disables or induces the degradation of the target RNA.
Who discovered antisense technology?
Introduction. The potential of oligodeoxynucleotides to act as antisense agents that inhibit viral replication in cell culture was discovered by Zamecnik and Stephenson in 1978 [[1]]. Since then antisense technology has been developed as a powerful tool for target validation and therapeutic purposes.
What is an example of gene silencing?
Solution : Gene silencing is a mechanism by which cells shut down large sections of chromosomal DNA. Gene silencing is done by incorporating the DNA to be silenced into a form of DNA called heterochromatin that is already silent….An example of gene silencing is:
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Which technique is used in gene silencing?
Gene silencing is a modern gene-editing technique used for genetic engineering experiments. Using techniques like RNA interference, CRISPR-CAS9 and antisense RNA technique, a gene of our interest can be suppressed or its expression is controlled.
What is the difference between antisense and siRNA?
Both platforms are intended to modulate gene expression. Both are nucleic acids and contain an antisense strand intended to recognize a target mRNA. They also have important differences. ASOs have one strand while siRNAs have two, a basic fact that may lower cost and simplify delivery.
What is antisense and sense?
Antisense DNA: DNA normally has two strands, i.e., the sense strand and the antisense strand. In double-stranded DNA, only one strand codes for the RNA that is translated into protein. This DNA strand is referred to as the antisense strand. The strand that does not code for RNA is called the sense strand.