How do you make carbol fuchsin solution?
How do you make carbol fuchsin solution?
Preparation of reagents. Carbol fuchsin (1%) was prepared from 10 g of basic fuchsin (Hi-Media) dissolved in 100 ml of methanol (Qualigens) and 50 ml of melted phenol (Qualigens) in a flask maintained at 60°C in a water bath. This solution was made up to 1,000 ml with distilled water.
What is the composition of carbol fuchsin?
Magenta II is a hydrochloride that is the monohydrochloride of 4-[(4-aminophenyl)(4-iminocyclohexa-2,5-dien-1-ylidene)methyl]-2-methylaniline. One of the minor constituents of Basic fuchsin, together with pararosanilin, rosanilin and new fuchsin. It has a role as a histological dye and a fluorochrome.
In which method carbol fuchsin stain is used?
Our Carbol Fuchsin (Ziehl-Neelsen) Stain is used in the microscopic detection of acid-fast microorganisms such as Mycobacterium. Acid-fast organisms such as Mycobacterium have cell walls that are resistant to conventional staining by aniline dyes such as the Gram stain.
How do you make basic fuchsin stain?
Dissolve 0.5 g basic fuchsin dye in 20 ml 95% ethanol. Dilute to 100 ml with distilled water. Filter if necessary with Whatman No. 31 filter paper to remove any undissolved dye.
Why is carbol fuchsin boiled?
The primary stain used in acid-fast staining, carbol fuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat in the form of heat (steam).
What is carbol fuchsin used for?
Carbol-fuchsin is used to treat postoperative phenol nail procedures. It may also be used as a first aid antiseptic drying agent in skin conditions where there is too much moisture. This medicine may also be used for other infections as determined by your doctor.
What is dilute carbol fuchsin?
Carbol Fuchsin (ZN, Dilute) is used as solution for monochrome staining of bacteria. Composition** Ingredients. Carbol fuchsin.
What is the role of phenol in carbol fuchsin?
The concentration of phenol is 5% according to WHO (7), IUATLD (3), and CDC (6) manuals. The authors diluted the 1% carbol fuchsin with water to make it 0.3% carbol fuchsin, which would also dilute the phenol concentration to ∼1.7%. The role of phenol is critical in the staining process (2).
Why is Carbolfuchsin used in acid-fast staining?
The primary stain used in acid-fast staining, carbolfuchsin, is lipid-soluble and contains phenol, which helps the stain penetrate the cell wall. This is further assisted by the addition of heat.
What reagents are used in the fluorescent method of AFB?
The acid-fast bacilli will stain bright red, and the background will stain blue. Reagents used in the procedure include Ziehl–Neelsen carbol-fuchsin solution, 1% acid alcohol, and methylene blue solution [15].
What is carbol Fuchsin used for?
What is fuchsin reagent?
Fuchsin Acid reagent is used as a component of the Mallory Trichrome kit. Mallory trichrome staining kit is used for treating the tested microscopic sample using three different stainings with differential counterstaining of two basic parts of the tissue (muscle and collagen fibers) in focus.