What is high cell density fermentation?

What is high cell density fermentation?

High cell density fermentation by fed-batch strategies is one of the most cost-effective means of achieving high yields for the production of large scale recombinant proteins in the bio-industry.

How do you increase cell density?

One approach to increase protein yield is to increase the total number of cells. In order to increase the number of cells, large bioreactors up to 25,000 liters would be used. A second approach is to increase the number of cells in the same volume, effectively increasing viable cell density.

What is maximum cell density?

Maximum cell densities ranged between 8.2 and 9.4 x 106 cells x mL-1 and represent the highest values described for High Five cells so far in the literature. They are 35% higher compared to those seen in pH-controlled and non-controlled experiments.

What is cell density?

The cell density (N) is defined as the number of cells or channels per unit of cross-sectional area perpendicular to the axis of the channel. From: Studies in Surface Science and Catalysis, 1998.

What is VCD cell culture?

In mammalian cell culture processes, viable cell density (VCD) is one of the most crucial parameters for monitoring of the current status of cell growth and protein production.

Why is cell density important?

Seeding the right cell density is necessary for optimum cell growth. Seeding a lower cell density will result in lack of cell to cell interaction and communication. As a result the cells will show slow growth and eventually die.

What is cell density in cell culture?

How does cell density affect cell growth?

Cell density is known to control the rate of proliferation of cells in culture (7, 28, 30). The transport of small nutrients is also dependent on cell density. It decreases when cell density increases and vice versa (9, 15, 18, 19, 20, 34).

What is viable cell density?

The Integral (area under the curve) of Viable Cell Density (IVCD) or Concentration (IVCC) is an essential calculated metric in cell culture operations. IVCC quantifies the effective working time for a dynamic viable cell concentration within a given frame of time, analogous to the calculation of man-hours.