What temperature does HindIII work best in and why?

Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer.

How many fragments pieces would you expect if you cut the linear lambda DNA with HindIII alone?

The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable for use as molecular weight standards for agarose gel electrophoresis (1).

How many DNA fragments will be produced if pBR322?

So the correct option is ‘4’.

How are bp fragments calculated?

To calculate the fragment size we simply need to subtract the bp difference between the two REs: 5198 bp – 5070 bp = 128 bp. DNA fragment 2 – a large fragment (and our desired DNA fragment as it contains all the features of the plasmid.

How do you cut DNA into fragments?

In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences. Different restriction enzymes recognise and cut different DNA sequences.

What cuts the backbone of the DNA?

This is the process of cutting out and replacing a single nucleotide. First, an enzyme called DNA glycosylase extracts the damaged part. Then an enzyme called AP endonuclease finds the gap in the sugar-phosphate backbone and removes neighbouring nucleotides.

Which chemicals are used to cut DNA into fragments?

In the laboratory, restriction enzymes (or restriction endonucleases) are used to cut DNA into smaller fragments. The cuts are always made at specific nucleotide sequences.

What does EcoRI cleave?

EcoRI (pronounced “eco R one”) is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.