What temperature does HindIII work best in and why?
What temperature does HindIII work best in and why? Thermo Scientific HindIII restriction enzyme recognizes A^AGCTT sites and cuts best at 37°C in R buffer. How many fragments pieces would you expect if you cut the linear lambda DNA with HindIII alone? The HindIII digest of lambda DNA (cI857ind1 Sam 7) yields 8 fragments suitable
Read More