What is slide agglutination test?

What is slide agglutination test?

A rapid slide agglutination test has been developed for the identification of Neisseria gonorrhoeae that are primarily detected as oxidase-positive colonies in gonococcal cultures. The technique is based on the specific nonimmune reactivity between the Fc portion of immunoglobulin (Ig)G and staphylococcal protein A.

What is the principle of agglutination test?

The reaction between a particulate antigen and an antibody results in visible clumping called agglutination. Antibodies that produce such reactions are known as agglutinins. The principle of Agglutination reactions are similar to precipitation reactions; they depend on the cross linking of polyvalent antigens.

What are agglutination test used for?

Agglutination tests are frequently used for initial confirmation of specific pathogens. Since antibodies to the target organism may cross-react with other organisms and autoagglutination may occur, these must be considered as screening tests and further confirmation will usually be necessary.

What is the process of agglutination?

Agglutination is the process that occurs if an antigen is mixed with its corresponding antibody called isoagglutinin. This term is commonly used in blood grouping. This occurs in biology in two main examples: The clumping of cells such as bacteria or red blood cells in the presence of an antibody or complement.

What are the types of agglutination?

There are two forms of agglutination. They are the active agglutination and the passive agglutination. With active agglutination, the antigen occurs naturally on particle. With passive agglutination, the antigen must first be bound to an inert particle to detect an antibody.

What is the meaning of slide test?

Widal test (slide method) helps to detect typhoid fever and paratyphoid fever which are collectively known as enteric fever. Enteric fever is caused by the ingestion of food or water which is contaminated by bacteria. This test is performed through the slide method.

What agglutination means?

Agglutination is defined as the formation of clumps of cells or inert particles by specific antibodies to surface antigenic components (direct agglutination) or to antigenic components adsorbed or chemically coupled to red cells or inert particles (passive hemagglutination and passive agglutination, respectively).

What does a positive agglutination test look like?

Examine the slide on 10x objective of your microscope or higher if needed. 6. With a negative result all the red cells can be seen freely floating apart from each other. With a positive result, clumps of red cells like bunches of grapes will be floating around interspersed by a few free cells.

What are the 5 types of agglutination?

Various methods of agglutination are used in diagnostic immunology and these incude latex agglutination, flocculation tests, direct bacterial agglutination, and hemagglutination.

Is agglutination positive or negative?

Agglutination accompanied by clearing of the background is seen in the positive reaction (A). There is no visible agglutination with the negative control latex reagent or in a negative test reaction (B).

What type of blood is agglutination?

Agglutination occurred when the RBC antigens were bound by the antibodies in the serum. He called the antigens A and B, and depending upon which antigen the RBC expressed, blood either belonged to blood group A or blood group B.